The Process Involved in the Extraction and Purification of DNA.
Deoxyribonucleic Acid, commonly referred to as DNA, is the molecule found in everything termed as living. The molecule contains genetic code of every organism thus a blueprint which acts as continuous plans for any substance that these organisms do produce at the course of their living. As much as the molecule has a popular usage, very few individuals have actually seen this molecule; actually the molecule happens to be too small for one to see. However, one can simply do extraction on the molecules easily at home or in the school using available household materials like plants and especially peas.
Some of the crucial materials that you may require for the process include alcohol, meat tenderizer, dishwashing detergent mostly in liquid form, a blender, a beaker or rather clear glass container, a stirring rod or a spoon. In the first procedure, blend at least a half cup of split peas in a cupful of water. With that, you should obtain a soupy mixture then use a homogenous solution between the mixtures. One may also consider reducing the mixture and spare some just in case. Then, pour some of the solutions you have, an adequate amount of it into a container up to a level of at least two inches.
The third procedure involves adding at least one tablespoonful of the liquid dishwashing detergent on top of the solution. Again stir the mixture slowly for about five minutes. In the next procedure; add a pinch of the meat tenderizer on the mixture then stir for another five minutes. After stirring the mixture, drizzle some alcohol above the container. Consider adding the alcohol until you obtain a layer of three inches on the split peas solution. Peas are particularly considered during the extraction because of their high source of this molecule. Again the detergent is used to break down the membrane cell for it to release its contents and the meat tenderizer to dissolve the membrane nuclear for it to release the DNA inside. The alcohol is used in the process to bring out the DNA strands.
During the purification process, there are a variety of processing kits that can be used. This simply is attributed by the fact that different samples work with different kind of tissues, forensic samples, plant parts and also different cellular structures. Endlessly, there are new inventions being done on machines and cutting edge products via forms of amplification and facilitations for use in new projects and experiments. One such way involves use of magnetized beads which happens to be very effective. Another effective way of purification involves the use of plasmid purification kits that enable the production of low endotoxin levels for use in the subject transfection.